Other
MTS

Part:BBa_K678025:Design

Designed by: DTU-Denmark-2   Group: iGEM11_DTU-Denmark-2   (2011-09-04)

Mitochondrial targeting sequence for Aspergillus nidulans


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 127
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 127
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 90
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 127
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 127
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This MTS should be placed at the N-terminal of the gene of interest.

This part was designed for the Plug'n'Play with DNA assembly standard (RFC 80), therefore this part contains tails at the 5' and 3' ends as shown on the figure below.

Source

Aspergillus nidulans genomic DNA (AN6279)

References

(1) Hynes MJ, Murray SL, Andrianopoulos A, Davis MA. (2011) Role of Carnitine Acetyltransferases in Acetyl Coenzyme A Metabolism in Aspergillus nidulans. Eukaryotic cell 10: 547-555.